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抗坏血酸促进盐胁迫条件下蚕豆种子萌发的机理研究

更新时间:2016-9-11:  来源:毕业论文

抗坏血酸促进盐胁迫条件下蚕豆种子萌发的机理研究 摘  要:以蚕豆种子“蚕豆2号”为材料,研究了0、0.5、1.0、1.5、2.0 mmol/L 5个浓度梯度的抗坏血酸(AsA)对盐胁迫(0.1mol/LNaCl)条件下蚕豆种子萌发机理及其抗氧化能力的影响,以探讨AsA调控种子萌发过程中响应盐胁迫的机理。结果显示:浓度为0.5mmol/L的AsA较明显的降低了盐胁迫下种子萌发过程中的丙二醛的含量,提高了种子的发芽率、发芽势、过氧化物酶和超氧化物歧化酶的含量以及淀粉酶的活力。结果说明,适宜浓度的AsA通过提高盐胁迫下抗氧化能力来促进蚕豆种子萌发,提高萌发种子的POD、SOD和淀粉酶的活力来维持植物体细胞膜的稳定性,降低膜脂过氧化伤害程度,从而缓解了盐胁迫对种子萌发的影响,以促进种子萌发。
关键词:抗坏血酸;盐胁迫;蚕豆;萌发;抗氧化

Study on Mechanism of Ascorbic Acid on the Promotion of Faba Bean Seeds Germination under Salt Stress
Abstract: In order to explore the mechanism of ascorbic acid (AsA) in the regulation of plant responsing to salt stress, the effects of 0, 0.5,1.0, 1.5, 2.0 mmol/L AsA on germina-
tion and antioxidant capacity of broad beans were studied. The results showed that: exog- enous 0.5 mmol/L AsA application on the seed significantly decreased the malondialdeh -yde content, while the germination vigor and rate, the activities of peroxidase, superoxide dismutase and amylase were increased. The results indicated that suitable concentration of AsA could enhance antioxidant capacity to promote seed germination and improve the POD, SOD and amylase activity of seeds to maintain the stability of plant cell membrane, reduce the degree of injury membrane lipid peroxidation, thus alleviating the injury caused by salt stress on seed germination and promoting seed germination.
Key words: Ascorbic Acid; Salt Stress; Vicia faba; Germinatiaon; Antioxidation.
目    录

摘要 1
Abstract 1
引言 2
1材料和方法 2
1.1材料培养及处理 2
1.2测定项目与方法 3
1.2.1形态指标 3
1.2.2生理指标 3
1.3数据分析 3
2结果与分析 3
2.1AsA对NaCl胁迫下蚕豆种子萌发的影响 3
2.2AsA对NaCl胁迫下蚕豆种子幼苗种子生长的影响 4
2.3AsA对NaCl胁迫下蚕豆种子萌发中生理特性的影响 5
2.3.1AsA对NaCl胁迫下蚕豆种子萌发中MDA含量的影响 5
2.3.2AsA对NaCl胁迫下蚕豆种子萌发POD含量的影响 5
2.3.3AsA对NaCl胁迫下蚕豆种子萌发SOD含量的影响 6
2.3.4AsA对NaCl胁迫下蚕豆种子萌发淀粉酶活力的影响 7
3讨论 8
参考文献 9
致谢 11,3032

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