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油菜核盘菌的分离鉴定及侵染分析

更新时间:2016-9-11:  来源:毕业论文

油菜核盘菌的分离鉴定及侵染分析 摘要:从河南省周口市采集感染菌核病的油菜,对染病油菜的核盘菌进行分离和鉴定。通过对菌落的显微形态观察,初步证明该菌株为S.sclerotiorum。对提取的核盘菌DNA进行核糖体DNA转录间隔区(ITS)扩增及进行化树分析,发现与已知核盘菌菌属ITS序列同源性达到100%,进一步证明该分离菌株为S.sclerotiorum。将配成一定浓度的培养好的核盘菌孢子悬浮液喷洒在实验室种植的番茄和拟南芥植株上,发现这两种植物均可被感染。
关键词:油菜;核盘菌;ITS序列;感染
Identification and Analysis Infection on Plants of Sclerotinia sclerotiorum on Oilseed Rape
Abstract:An strain of Sclerotinia sclerotiorum was isolated from oilseed rape (Brassica napus) in Zhoukou city of Henan province with the method of single-spore isolation.The morphology and microscopic analysis indicated that the isolated stain was S. sclerotiorum. The extracted DNA of Sclerotinia sclerotiorum was sequenced and analyzed, which showed 100% homology to ITS sequences of some S. sclerotiorum stains. The tomato and Arabidopsis plants were sprayed by the cultivation of a certain concentration of Sclerotinia sclerotiorum spore suspension, which found that they all can be infected.
Key words: Napus; Sclerotinia sclerotiorum;ITS; Infection
   目    录

摘要 1
关键词 1
引言 1
1.实验材料 2
1.1 供试材料 2
1.2 供试培养基 2
1.3 供试试剂 2
2.实验方法 3
2.1 核盘菌的显微形态分析 3
2.2 核盘菌ITS序列分析 3
2.2.1 核盘菌DNA的提取 3
2.2.2 PCR的扩增和检测 3
2.3 核盘菌的侵染实验 3
2.3.1 核盘菌侵染植物叶片 3
2.3.2 DAB和台盼蓝同时染色法 4
3.结果与分析 4
3.1 油菜菌核病病原菌的形态特征 4
3.2 ITS区的扩增和测序 5
3.3 拟南芥植物和番茄植物病原致病性分析 5
4.讨论 6
参考文献 7
致谢 9,3033

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