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软骨中硫酸软骨素的提纯与应用

时间:2018-10-11 21:08来源:毕业论文
以牛软骨作为原料,从中提取硫酸软骨素的多糖的碱法提取工艺和硫酸软骨素的酶法提取工艺。硫酸软骨素的测定方法、多糖组分分析以及软骨硫酸软骨素的应用

摘要:硫酸软骨素是一种天然酸性粘多糖,属于生物高分子化合物,英文全称为Chondroitinsulfate,简称CS。硫酸软骨素存在三种异构体,分别是A和C以及D,这三种异构体都是由D2葡萄糖醛酸和N2乙酰2D2氨基半乳糖硫酸酯为单位组成的,只是硫酸基的位置不同。硫酸软骨素通过共价键与核心蛋白相结合,普遍存在于人和一些动物的软骨组织中,比如牛、鸡、鱼等等,。同时硫酸软骨素具有降血脂、抗凝血、抗肿瘤和心血管硬化、促进冠状动脉循环等活性等生理作用 ,是治疗风湿和类风湿疾病的重要药物,对冠心病、动脉粥样硬化、心肌梗塞等心血管疾病的防治有一定效果。也被用于食品,化妆品等领域。28987
实验以牛软骨作为原料,从中提取硫酸软骨素的多糖的碱法提取工艺和硫酸软骨素的酶法提取工艺。硫酸软骨素的测定方法、多糖组分分析以及软骨硫酸软骨素的应用。
结果显示,提取硫酸软骨素的最佳酶解条件为120U/mL的酶浓度,料液比为
1:12,50摄氏度条件下酶解25h。此条件下硫酸软骨素的得率达13.28 。
实验以阴离子交换树脂进行硫酸软骨素的纯化,通过旋转蒸发浓缩得到硫酸软骨素纯品。纯度提高了25.7%,为53.4%,而得率下降了16%,为11.14%。
本次实验将硫酸软骨素应用于乳膏剂中,使硫酸软骨素除口服之外增加了外敷的功能。
论文主要研究了硫酸软骨素的提纯与应用,研究结果可对硫酸软骨素的制备与应用提供参考数据。
毕业论文关键词:硫酸软骨素;提取;纯化;硫酸软骨素乳膏剂 源自<六~维:论"文>网.加7位QQ3249`114 www.lwfree.cn
Purification and application of chondroitinsulfate in the cartilage
Abstract: Chondroitinsulfate (CS) is a kind of natural polysaccharide, belonging to biological macromolecular compounds. CS has 3 isoforms of A, C and D, which are composed of D2 grape sugar acid and N2 acetyl 2D2 amino group galactose, but the position of the sulfate is different..CS is widely found in cartilage tissues such as pigs, cattle, sheep and fish, and so on. And the combination of covalent bonds and core proteins.. CS has promoting coronary circulation, reducing blood lipid, anti coagulation, anti tumor and cardiovascular sclerosis activity, mainly is the medicine for treating rheumatism and rheumatoid disease, prevention and treatment of coronary heart disease, atherosclerosis, myocardial infarction and cardiovascular disease have certain curative effect . Also used in food, cosmetics and other fields.
Experiments with bovine cartilage as a raw material, the extraction of chondroitin sulfate polysaccharides by alkali extraction process and chondroitin sulfate enzymatic extraction process. The method, determination of chondroitin sulfate polysaccharide component analysis and application of chondroitin sulfate.
The results showed, hydrolysis conditions were enzyme concentration 120U/mL enzyme optimum extraction of chondroitin sulfate, liquid material ratio 1:12, 50 degree Celsius conditions of the hydrolysis of the 25h. The yield reached 13.28 chondroitin sulfate under this condition.
The experiment of purification of anion exchange resin for chondroitin sulfate, chondroitin sulfate was purified by rotary evaporation. The purity increased by 25.7% to 53.4%, while the yield decreased by 16%, 11.14%.
The experiment will be applied to the cream of chondroitin sulfate, chondroitin sulfate in addition to the oral external use function.
Paper focuses on the study of the chondroitin sulfate purification and application. The results of this study of chondroitin sulfate of preparation and application to provide reference data. 源自<六~维:论"文>网.加7位QQ3249`114 www.lwfree.cn
Key words: Chondroitinsulfate;Abstract;Purification;Cream of CS
目录
1 绪论    1
1.1 硫酸软骨素概述    1
1.2 硫酸软骨素的作用    1
1.3 硫酸软骨素的提取工艺和方法    2 软骨中硫酸软骨素的提纯与应用:http://www.lwfree.cn/shengwu/20181011/24057.html
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