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桑叶中1-脱氧野尻霉素(DNJ)的提取与检测

时间:2017-09-26 15:24来源:毕业论文
建立DNJ定性定量方法,结合薄层色谱法对DNJ快速定性,结合乙酰化气相色谱法检测DNJ含量,为DNJ富集生产提供工间检测手段。主要研究内容和结论如下:1、研究了DNJ的TLC分析方法
摘要:本课题对建立相对比较完善的1-脱氧野尻霉素的定性定量方法作研究。桑叶常用于中药的合成中,其中的DNJ被认为是一种高效的α-糖苷酶抑制剂,具有很好的降血糖活性,应用前景广阔。本次建立DNJ定性定量方法,结合薄层色谱法对DNJ快速定性,结合乙酰化气相色谱法检测DNJ含量,为DNJ富集生产提供工间检测手段。主要研究内容和结论如下:1、研究了DNJ的TLC分析方法。采用高效硅胶G薄层板,正丙醇:醋酸:水 = 4:1:1为展开剂,碱性高锰酸钾试剂显色,结果显示DNJ可以与桑叶中的其他成分分离开来,比移值是0.57。此法简单灵敏,重现性好。2、研究了DNJ的GC分析方法。采用乙酰化衍生气相色谱法分析测定了桑叶中DNJ的含量,实验测得:一些市场上购买的DNJ标品的含量如下:fc公司DNJ含量为100%(产品标出含量为≥98%),Sigma公司DNJ•HCl含量为98%(产品标出含量为99%),华汉公司DNJ含量为75.8%(产品标出含量为≥95%),研生公司DNJ含量为86.2%(产品标出含量为≥98%),华汉公司的DNJ含量为6.3%(产品标出含量为≥10%)。江苏滨海地区产的桑叶样品经纯化后,在经过乙酸酐乙酰化,进入气相色谱分析。线性范围为0.01-0.1mg/mL,(相关系数r=0.9999)。实验测得桑叶中DNJ含量为0.040%。并且进行了精密度试验,结果显示RSD为2.0%(n=5)。13740
关键词:1-脱氧野尻霉素;桑叶;薄层色谱;气相色谱法
 Extraction and Detection of 1-deoxynojirimycin (DNJ) from Mulberry Leaves
Abstract: The subject is devoted in establishing the qualitative and quantitative methods of 1-deoxynojirimycin (DNJ) from mulberry leaves.Mulberry leaves,is commonly used in the synthesis of traditional Chinese medicine (TCM),the DNJ of which is considered to be an efficient alpha-glucosidase inhibitor with good hypoglycemic activity and broad application prospects.The establishment of DNJ qualitative and quantitative methods, combined with thin-layer chromatography and gas chromatography after acetylation to detect the content of DNJ,Which can provide workers the detection means in the DNJ enrichment production process.The main contents and conclusions were as follows:1.The TLC method was established to analysize DNJ.Efficient silica gel G plates, N-propanol: acetic acid: water = 4:1:1 as developing solvent,the Alkaline permanganate solution coloration.The result showed that DNJ can be separated from other compositions,the Rf is 0.57.The method was easy,sensitive with good reproducibility.2.A gas chromatography method for the determination of DNJ from mulberry leaves was established after acetylation.The results showed that DNJ content of standard products purchased on the market are as follows:the fc company DNJ content of 100% (Products marked content≥98%),Sigma Corporation DNJ-HCl content of 98% (Products marked content≥99%),pioneer Herb Industrial co,Ltd DNJ content of 75.8% (Products marked content≥95%),Yansheng company DNJ content of 86.2% (Products marked content≥98%),pioneer Herb Industrial co,Ltd DNJ content of 6.3% (product marked content≥10%).Mulberry leaf samples from Jiangsu coastal area were purified,then derivatized by acetic anhydride for gas chromatography analysis.The method showed good linear relationship in the range of 0.01-0.1mg/mL (correlation coefficient R = 0.9999).DNJ content in mulberry leaves was 0.040%.In the optimized conditions, repetitive test was performed, the RSD of repetitive test was 2.0% (n=5).

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KeyWords:1-deoxynojirimycin (DNJ),mulberry leaves,thin-layer chromatography (TLC),gas chromatography.
 目录
1  前言    1
1.1  研究的意义与目的    1
1.2  1-脱氧野尻霉素(DNJ)的简介    2
1.3  薄层色谱    3
1.3.1  分析原理    3
1.3.2  分析特点    3 桑叶中1-脱氧野尻霉素(DNJ)的提取与检测:http://www.lwfree.cn/yixue/20170926/14072.html
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