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青菜中3种邻苯二甲酸酯及其单酯代谢物的残留动态研究

时间:2017-10-09 16:11来源:毕业论文
在青菜培养细胞中的PAEs和单烷基邻苯二甲酸酯(MPEs)表明DBP的水解速度比DEHP来的迅速,而单酯的转化速度比其前体更快。考虑到PAEs转化到单酯的进程在整个植物和植物细胞中广泛存在
摘要:邻苯二甲酸酯类(PAEs)是一种普遍存在于地膜和废物处理中的大体积化学品。蔬菜之类的食品可能通过土壤吸收并累积PAEs,使得人类的膳食过程中存在隐藏的健康风险。本实验中我们进行了青菜的栽培研究,以确定植物吸收、转运和代谢邻苯二甲酸二(2-乙基)己酯(DEHP)和邻苯二甲酸二丁酯(DBP)及其主要代谢产物邻苯二甲酸单(2-乙基)己酯(MEHP)和邻苯二甲酸单丁酯(MBP)的能力。所有这四种在植物生物体中被检测到的化合物,其生物富集系数的范围均在0.16 ± 0.01 到 4.78 ± 0.59。然而,实验因子却很难从根部转移到叶子中去,因此叶片中的易位因子低于1。此外,PAEs在被吸收之后很容易会被转换成其单酯代谢物。在青菜培养细胞中的PAEs和单烷基邻苯二甲酸酯(MPEs)表明DBP的水解速度比DEHP来的迅速,而单酯的转化速度比其前体更快。考虑到PAEs转化到单酯的进程在整个植物和植物细胞中广泛存在,我们认为这些单酯化合物如MPEs应该在评估人类通过吃被PAEs污染过的土种出来的菜时一同纳入测量。14031
关键词: 青菜;邻苯二甲酸酯;富集系数
The residual dynamics of three phthalate esters and their metabolites on pakchoi
Abstract: Phthalate esters (PAEs) are bulk chemicals and are found ubiquitously in soil as a result of widespread plasticulture and waste disposal. Food plants such as vegetables may take up and accumulate PAEs from soil, potentially imposing human health risks through dietary intake. In this study, we carried out a cultivation study using pakchoi to determine the potential of plant uptake, translocation, and metabolism of di(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP) and their primary metabolites mono(2-ethylhexyl) phthalate (MEHP) and monobutyl phthalate (MBP). All four compounds were detected in the plant tissues, with the bioconcentration factors (BCFs) ranging from 0.16 ± 0.01 to 4.78 ± 0.59. However, the test compounds were poorly translocated from roots to leaves, with a translocation factor below 1. Further, PAEs were readily transformed to their monoesters following uptake. Incubation of PAEs and monoalkyl phthalate esters (MPEs) in cell culture showed that DBP was hydrolyzed more rapidly than DEHP, while the monoesters were transformed more quickly than their parent precursors. Given the extensive metabolism of PAEs to monoesters in both whole plants and plant cells, metabolism intermediates such as MPEs should be considered when assessing human exposure via dietary intake of food produced from PAE-contaminated soils.
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Key words: pakchoi; phthalate esters; bioconcentration factors
目录    4
1 绪论    5
1.1  实验背景    5
1.2  研究目标    6
1.3  研究意义    7
1.4  PAEs塑化剂的概述    7
1.5  研究方法与技术路线    8
2 实验方法    9
2.1  青菜中邻苯二甲酸酯及其代谢产物检测方法的建立    9
2.2  QuEChERS -GC-MS/MS法测定蔬菜中邻苯二甲酸酯及其代谢产物    11
2.2.1 实验部分    13
2.3  青菜在生长过程中对邻苯二甲酸酯及其代谢产物的积累和代谢的研究    14
2.3.1 实验用土壤的老化试验    14
2.3.2 盆栽的材料和方法    14
2.3.3 蔬菜和土壤中PAEs和MPEs的检测方法    15
2.3.4 土壤中PAEs的检测方法    15
2.3.4.1土壤样品的处理:    15
2.3.4.2 GC-MS仪器条件    16
2 实验结果分析    16
3.1  QuEChERS -GC-MS/MS法测定青菜中2种邻苯二甲酸酯的结果分析    16 青菜中3种邻苯二甲酸酯及其单酯代谢物的残留动态研究:http://www.lwfree.cn/yixue/20171009/14446.html
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