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甾体药物中间体生物转化条件的优化

时间:2018-04-28 09:44来源:毕业论文
通过对不同配方的选择,流动相,有机溶剂和纯化溶剂的优化来提高4-雄(甾)烯-3,17-二酮的生成量,抑制中间体的生成,得到高产的4-雄(甾)烯-3,17-二酮,从而提高其转化率。实验所采取的

摘要:本实验的目的是通过对不同配方的选择,流动相,有机溶剂和纯化溶剂的优化来提高4-雄(甾)烯-3,17-二酮的生成量,抑制中间体的生成,得到高产的4-雄(甾)烯-3,17-二酮,从而提高其转化率。实验所采取的方法是将植物甾醇通过种子培养基,发酵培养基,萃取,浓缩,纯化,离心等步骤转化为4-雄(甾)烯-3,17-二酮。通过高效液相色谱法,对4-雄(甾)烯-3,17-二酮进行含量测定,然后通过计算得到转化率。采取甲醇溶液作为其纯化溶剂时,4-雄(甾)烯-3,17-二酮在配方Ⅴ的条件下转化率为41.2%,在5种配方中转化率较大,但没有达到理想的转化率值。采取乙酸乙酯作为其纯化溶剂时,4-雄(甾)烯-3,17-二酮在配方Ⅴ的条件下转化率为84.3%,为5个配方中转化率最大,并达到了理想的转化率值。21924
毕业论文关键词:甾体化合物;植物甾醇;生物转化;4-雄(甾)烯-3,17-二酮(AD);高效液相色谱法
Opitmization of transformation conditions of steroid drug intermediates

Abstract: The purpose of this experiment is to select different formulation by its mobile phase, an organic solvent and purified solvent to optimize the increase the amount of androst-4-ene-3,17-dione , inhibit the formation of intermediates obtained yield androst-4-ene-3,17-dione, thereby improving the conversion rate. The experiment method is to take the seed through the medium of phytosterols, fermentation medium, extraction, concentration, purification, and centrifugation step was converted to androst-4-ene-3,17-dione. By high-performance liquid chromatography of androst-4-ene-3,17-dione to determine the content, then get the conversion rate by calculation. Taking methanol as the solvent was purified androst-4-ene-3,17-dione  in the formulation under conditions Ⅴ conversion was 41.2% and the conversion rate is high in the five kinds of formulations, but did not reach the desired conversion values. Taking ethyl acetate as the solvent was purified androst-4-ene-3,17-dione in the formulation under conditions Ⅴ conversion was 84.3%, the conversion of the formula 5 maximum and reach the ideal conversion values. 源自L六^维:论`文~网-.加7位QQ3249`114 www.lwfree.cn
Keywords : steroids ; phytosterols ; biotransformation ; Androstenedione (AD); HPLC
目  录
1 研究背景    1
1.1甾体化合物及甾体药物    1
1.2 甾体化合物的微生物转化    2
1.3 植物甾醇及甾醇侧链降解    2
1.3.1 植物甾醇    3
1.3.2 侧链降解产物AD    3
1.4 雄甾-4-烯-3,17-二酮的分析方法    4
1.4.1 紫外分光光度法    4
1.4.2 气相色谱法(GC)    4
1.4.3 高效液相色谱法(HPLC)    5
1.5 本课题的研究意义及研究内容    5
1.5.1研究意义    5
1.5.2 研究内容    5
2 实验材料及方法    6
2.1 实验材料    6
2.1.1 原材料    6
2.1.2 主要试剂    6
2.1.3 主要仪器    7
2.2 实验部分    7
2.2.1 种子培养基的配制    7
2.2.2 发酵培养基的配制    8
2.2.3 种子培养基的接种    8
2.2.4 发酵培养基的接发酵    8
2.2.5 流动相的选择    8
2.2.6 有机溶剂的选取    8
2.2.7 纯化溶剂的选择    8
2.2.7.1 选用乙酸乙酯作为纯化溶剂    8
2.2.7.2 选用甲醇溶液作为纯化溶剂    9
2.2.8 取样    9
2.2.9 雄烯二酮标准曲线的绘制    9
2.2.10 分析检测    9 甾体药物中间体生物转化条件的优化:http://www.lwfree.cn/yixue/20180428/14344.html
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